Effects of the grub extract on apoptosis of MCF-7 human breast cancer cell line
- VernacularTitle:蛴螬提取物对MCF-7人乳腺癌细胞株凋亡的影响
- Author:
Hua JIN
;
Shu SUN
;
Baiyan YU
;
Wanshan YANG
;
Tiefeng JIN
- Publication Type:Journal Article
- Keywords:
Grub extract;
MCF-7 cells;
Apoptosis;
Protein Bcl-2;
Protein Fas;
Caspases
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the apoptotic pathway of MCF-7 breast cancer induced by the grub extract in vitro.METHODS:MTT assay was used to determine the effect of the grub extract on proliferation of MCF-7 human breast cancer cell line and cell toxicity.Morphological changes of the apoptosis in cancer cells were observed by HE staining through invert microscope,light microscope,AO/EB double fluorescent staining under fluorescent microscope.FCM was used to assay the change of apoptotic rate.The expression of Bcl-2,Fas,caspase-9,caspase-3 in apoptotic pathway was detected with immunocytochemical method before and after exposure to the grub extract,and the effect of that on apoptotic pathway was explored.RESULTS:(1)The MTT test showed that the growth of MCF-7 human breast cancer cell line was significantly inhibited by the grub extract in dose and time dependent manners.The inhibitory rate in exposure group was significantly different from that in control group(P
