Lysophosphatidic acid (LPA) induces the proliferation of astrocytes in rat via pathway of protein kinase C-? and calcium ion in vitro
- VernacularTitle:溶血磷脂酸通过蛋白激酶C-?/钙离子途径促进大鼠星形胶质细胞增殖
- Author:
Na GAN
;
Fei YIN
;
Jing PENG
;
Hongyuan ZHANG
- Publication Type:Journal Article
- Keywords:
Astrocytes;
Lysophospholipids;
Protein kinase C;
Cell proliferation;
Calcium
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To determine if lysophosphatidic acid(LPA)regulates the proliferation of astrocytes(AS)and to approach the mechanism of the process.METHODS:The cerebral AS of the neonatal SD rats were cultured in vitro and divided randomly into control group,PKC excitomotor(PMA)group,LPA group,PKC-? inhibitor(Ro31-8220)group,Ro31-8220+PMA group and Ro31-8220+LPA group.The proliferation of the cells was detected by MTT assay and flow cytometry(FCM).The concentration of intra-cellular calcium ion of the cells([Ca~(2+)]_i)which were labeled with Fura-2/AM was determined by ultraviolet spectrophotometer.The change of PKC-? inside the cells was observed by Western blotting.RESULTS:LPA and PMA stimulated the proliferation of AS,they also enhanced the expression of PKC-? and increased the concentration of [Ca~(2+)]_i.After pretreated with Ro31-8220,the abilities of LPA that mentioned above were decreased.The change of [Ca~(2+)]_i was associated with the diversity of PKC-?.CONCLUSION:LPA promotes the proliferation of AS via the way of PKC-? and Ca2+.
