Upregulative effect of CGRP on expression of CREB and phospho-CREB protein during focal cerebral ischemia/reperfusion in rat parietal cortex
- VernacularTitle:CGRP对局灶性脑缺血再灌注大鼠顶叶皮质CREB和p-CREB的上调作用
- Author:
Zhenghong ZHANG
;
Hongju XIAO
;
Gangming XI
;
Peng QU
;
Xiubin FANG
- Publication Type:Journal Article
- Keywords:
Brain ischemia;
Calcitonin gene-related peptide;
c-AMP response element binding protein;
Rats;
Parietal lobe
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of calcitonin gene related peptide(CGRP)on the expression of cyclic AMP response element binding protein(CREB)and phosphorylated-CREB in rat parietal cortex during focal cerebral ischemia/reperfusion(I/R).METHODS:Focal cerebral ischemia/reperfusion model was induced by occlusion of the right middle cerebral artery using the intraluminal suture method.The expressions of CREB and phospho-CREB in the parietal cortex in different groups(sham group,focal cerebral ischemia/reperfusion group and CGRP group)were detected with immunohistochemistry and Western blotting,and the positive products were analyzed by image analysis system.RESULTS:There was definite expression of CREB in right parietal cortex in sham group,while it was lesser in I/R group than that in sham group,but it became more in CGRP group than that in I/R group(P
