Anti-angiogenic Activity of Ray Cartilage Glycosaminoglycans:An Empirical Study
- VernacularTitle:魟鱼软骨多糖抗血管形成的实验研究
- Author:
Bin GUO
;
Guanying HAN
- Publication Type:Journal Article
- Keywords:
Ray cartilage glycosaminoglycans;
Human umbilical vein endothelial cell;
Rats;
Mice;
Corneal neovascularization;
Microvessel density
- From:
China Pharmacy
1991;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the anti-angiogenic activity of ray cartilage glycosaminoglycans(RCG).METHODS:Primary culture of human umbilical vein endothelial cells(HUVEC)was performed,and MTT was adopted to evaluate the effects of RCG on the proliferation of HUVEC;Corneal neovascularization(CNV)was induced by sutures on Wistar rats,and CNV area was calculated to evaluate the effects of different concentration of RCG on CNV in Wistar rats.The model of mice with Lewis lung carcinoma was made to observe the effects of different concentration of RCG on tumor growth,with inhibition ratio of primary tumor measured and microvessel density(MVD)quantitated by immunohistochemical staining.RESULTS:RCG obviously inhibited the proliferation of HUVEC in vitro,with IC50 value at 62.93 mg?mL-1.As compared with normal saline group,RCG groups showed smaller areas of new blood vessels(P
