Topiramate Promotes Proliferation of Neural Stem Cells in Vitro
- VernacularTitle:托吡酯促进体外培养神经干细胞增殖的实验研究
- Author:
Xiaohu ZHU
;
Xiaping CHEN
;
Qibin WANG
;
Junhua WANG
- Publication Type:Journal Article
- Keywords:
Topiramate;
Neural stem cells;
Proliferation;
MTT method
- From:
China Pharmacy
1991;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of topirmate on proliferation of neural stem cells in vitro.METHODS:Primary neural stem cells from newborn mice were cultured.The neural stem cells were incubated together with topiramate at 0.4,2 or 10 ?mol?L-1.A vehicle control and positive control were set up,respectively.The quantity and viability of neural stem cells newly generated were analyzed by a convert phase microscope,the neurosphere numbers were counted,and neural stem cells viability was determined and a MTT assay.RESULTS:The number of neurospheres of groups treated with topiramate at 2 and 10 ?mol?L-1 are 20.67?4.04,49.68?3.79,respectively,and the value of MTT assay of the two groups are 0.354 6?0.070 5,0.501 8?0.036 9,it was higher than the vehicle control neural stem cells(7.34?2.31、0.288 0?0.009 3,P
