Determination of L-Arginine Content in Cefradine for Injection by HPLC
- VernacularTitle:HPLC法测定注射用头孢拉定中L-精氨酸的含量
- Author:
Jiaai YANG
;
Hui WU
;
Bin QIAN
;
Jun ZHANG
- Publication Type:Journal Article
- Keywords:
Cefradine for injection;
L-arginine;
HPLC;
Content determination
- From:
China Pharmacy
1991;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method for the determination of the content of L-arginine in cefradine for injection.METHODS:The chromatographic analysis was carried on LiChrospher Diol column,and the mobile phase was composed of 0.01 mol?L-1 ammonium dihydrogen phosphate buffer solution(adjusted pH to 2.0?0.1 with phosphoric acid)-acetonitrile(25∶75)at a flow rate of 1.0 mL?min-1;the column temperature was 30 ℃;the detection wavelength was 206 nm,and the sample size was 20 ?L.RESULTS:The linear range of L-arginine was 51.2～307.2 ?g?mL-1(r=0.999 9).The average recovery was 100.7%(RSD=0.6%).CONCLUSION:The method is rapid,accurate and simple,and suitable for the determination of L-arginine in cefradine for injection.
