Determination of Serum Concentration of Deoxyschisandrin by RP-HPLC
- VernacularTitle:RP-HPLC法测定五味子甲素的血药浓度
- Author:
Yu AN
;
Juan LI
;
Yan DING
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Deoxyschisandrin;
Serum drug concentration
- From:
China Pharmacy
2007;0(36):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the serum concentration of Deoxyschisandrin by RP-HPLC.METHODS:The chr-omatographic separation was performed on Phenomenex C18(250mm?4.6mm,5?m) column with column temperature at 30℃.The mobile phase consisted of methanol - water (80∶20) at a flow rate of 1mL?min-1. The detection wavelength was set at 252nm. RESULTS:The calibration curve of Deoxyschisandrin was linear in the concentration range from 0.52 to 33.28?g?mL-1 (r=0.999 6). The average recovery of deoxyschisandrin was (97.27?4.11)%(RSD=4.23%,n=9).CONCLUSION:The method is simple, accurate and sensitive, and it can be used for the determination of serum concentration of Deoxyschisandrin.
