Quantitative Determination of Peoniflorin in Tiaojingquban Tablets by RP-HPLC
- VernacularTitle:RP-HPLC法测定调经祛斑片中芍药苷的含量
- Author:
Ping LIU
- Publication Type:Journal Article
- Keywords:
Tiaojingquban tablets;
HPLC;
Peoniflorin;
Quantitative determination
- From:
China Pharmacy
2007;0(36):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a RP-HPLC method for the determination of Peoniflorin in Tiaojingquban tablets. METHODS:The chromatographic separation was performed on VP-ODS column (150mm?4.6mm, 5?m). The mobile phase consisted of methanol-water (25∶75) at a flow rate of 1.0mL?min-1.AUV-detector at 230nm was used. RESULTS:The linear range of Peoniflorin was 24.8~198.4?g?mL-1(r=0.999 9).The mean recovery was 99.36% with RSD being 0.86% (n=6).CONCLUSION:The method is simple, rapid, sensitive and reproducible, and it can be used for the quality control of Tiaojingquban tablets.