Effects of cinnamyl aldehyde on c-Fos and c-Myc expression in NIH3T3 cells
- VernacularTitle:桂皮醛对NIH3T3细胞c-Fos、c-Myc表达的影响
- Author:
Jingxia ZHAO
;
Ping LI
;
Qifu HUANG
;
Xin LIU
;
Xun SHENG
;
Daiying LIANG
- Publication Type:Journal Article
- Keywords:
Cinnamyl aldehyde;
Cell proliferation;
Protein c-Fos;
Protein c-Myc;
NIH3T3 cells
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:Cinnamyl aldehyde (CA) is one alcohol ingredient derived from Cinnamomum cassia,which is widely used in treating chronic skin wound in Chinese medicine with the curative effect of ‘rescuing YANG’. The purpose of the present study was to investigate the expression of c-Fos, c-Myc proteins at different time points in NIH3T3 treated with CA and explore the possible mechanism of promoting cell proliferation by CA. METHODS: MTT assay was used for observing cell proliferation. Expression of c-Fos and c-Myc proteins in NIH3T3 cells were assessed by immunocytochemistry assay. RESULTS: The cell proliferation was promoted obviously when CA concentration was between 8.8?10-2 ?g/L and 8.8?10 ?g/L. CA at concentration of 5.5 ?g/L significantly induced expression of c-Fos, c-Myc proteins at 2-3 h after the stimulation compared with control group (P
