Changes of [Ca~(2+)]_i and the activity of ACE in alveolar macrophages of rabbits with high-fat diet
- VernacularTitle:高脂饮食兔肺泡巨噬细胞[Ca~(2+)]_i及ACE活性变化
- Author:
Jing ZHANG
;
Mingli TU
;
Shangjie WU
- Publication Type:Journal Article
- Keywords:
High-fat diet;
Macrophages;
Angiotensin converting enzyme;
Calcium;
Rabbits
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of high-fat diet on the level of intracellular free calcium ([Ca2+]i) and the activity of angiotensinⅠconverting enzyme (ACE) in alveolar macrophages (AMs) of rabbits. The association between asthma and high-fat diet was also observed. METHODS: Twelve male New Zealand rabbits were medially divided into normal diet group and 1.2% high-cholesterol diet group randomly. 8 weeks later, bronchial alveolar lavage was performed in vitro. [Ca2+]i was determined by Fluo-2/am.The activity of ACE was detected with ultraviolet method. RESULTS: The levels of [Ca2+]i in AMs greatly increased (P
