Effect of homocysteine-mediated endoplasmic reticulum stress on lipid metabolism in hepatocytes
- VernacularTitle:同型半胱氨酸介导的内质网应激对肝细胞脂质代谢的影响
- Author:
Chungen YAN
;
Guangyuan REN
;
Dongfang ZHU
;
Peihua NI
;
Huijuan ZHOU
- Publication Type:Journal Article
- Keywords:
Homocysteine;
Endoplasmic reticulum;
Stress;
Lipid metabolism
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the effect of homocysteine (Hcy)-mediated endoplasmic reticulum stress on lipid metabolism in hepatocytes.METHODS: HepG2 cells used in the study were treated with 5 mmol/L Hcy. The concentrations of Hcy, triglycerides (TGE) and cholesterol (CHO) in the cells were measured. In high methionine diet-induced hyperhomocysteinemia C57BL/6 mice, the concentrations of TGE and CHO in hepatocytes were analyzed. The mRNAs and proteins expressions of glucose-regulated protein 78 (GRP-78) and sterol regulatory element binding protein (SREBP-1) were also assessed.RESULTS: The concentrations of Hcy and lipids (TGE, CHO) in HepG2 cells at different time point were elevated after treated with 5 mmol/L Hcy (P0.05,vs 0 week). The mRNAs and proteins expressions of GRP-78 and SREBP-1 in mice at different time point after high methionine diet were higher than that at 0 week (P
