Effect of advanced glycation end products on inflammation in cultured cardiomyocytes
- VernacularTitle:糖基化终产物诱导心肌细胞炎症反应的研究
- Author:
Bo HU
;
Xiaogang ZHANG
;
Linlin ZENG
- Publication Type:Journal Article
- Keywords:
Cardiomyocytes;
Glycation end products, advanced;
Inflammation
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of advanced glycation end products on inflammation in cultured cardiomyocytes.METHODS: Primary cardiomyocytes were isolated from Sprague-Dawley neonatal (1 to 2 days old) rats ventricles. The insulin resistant cardiomyocyte model was established. Neonatal rat ventricular myocytes were exposed to AGEs for 24 hours. TNF-? mRNA and PPAR-? mRNA expressions were determined by RT-PCR. Activation of NF-?B in the cells was examined by using immunocytochemistry. The ultrastructure of the cells was detected by transmission electron microscope.RESULTS: The exprssion of TNF-? mRNA and the activation of NF-?B increased, the expression of PPAR-? mRNA decreased compared with control group (P
