The influence of RhoC on the expression of angiogenic factors in HCC cells
- VernacularTitle:RhoC基因对肝癌细胞促血管生长因子表达的影响
- Author:
Yansong ZHENG
;
Xinsheng LU
- Publication Type:Journal Article
- Keywords:
Carcinoma,Hepatocellular;
Genes,RhoC;
Gene Expression;
VEGF;
bFGF
- From:
Chinese Journal of General Surgery
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of pcDNA3.1-RhoC on the expression of endogenous angiogenic factors in HCC cells.Methods The reconstructed plasmid pcDNA3.1-RhoC was transfected into HepG2 cells, and expression of VEGF and bFGF was detected with the RT-PCR and immunohistochemical stain. HepG2 cells transfected with pcDNA3.1-RhoC or pcDNA3.1 were implanted into nude mice to observe the tumor occurrence rate.Results HepG2 cells transfected with pcDNA3.1/RhoC showed higher expression of RhoC . The expression of RhoC enhanced the expression of VEGF and bFGF(P
