Neural Stem Cells Combined with Olfactory Ensheathing Cells Improve the Recovery of Motor Function in Rats Subjected to Spinal Cord Transected Injury
- VernacularTitle:神经干细胞与嗅鞘细胞移植对脊髓全横断大鼠后肢运动功能的影响
- Author:
Jinwei YANG
;
Huijuan YANG
;
Luwei YIN
;
Jia LIU
;
Liyan LI
;
Tinghua WANG
- Publication Type:Journal Article
- Keywords:
Spinal cord injury;
Neural stem cells;
Olfactory ensheathing cells;
Rat
- From:
Journal of Kunming Medical University
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the engrafted effect of NSCs combined with OECs on the motor function repair of hindlimbs in rats subjected to spinal cord transected injury.Methods The NSCs and OECs were isolated from the Transgenic Green Fluorescent Protein(GFP) embryo mouse' hippocamp and neonatal mouse' olfactory bulb.Rats were divided into NSCs group,OECs group and co-transplantation group as well as control group.The recovery of posterior limb motor function in rats subjected to spinal cord transected injurywas evaluated by the BBB locomotion score respectively in each weekend among eight weeks.This was followed by a histochemiscal observation to know the engrafted cells survival and migration in the spinal cord of host under the fluorescence microscope.Results The NSCs and OECs cells survived and migrated in the spinal cord of transplantation groups:the motor functional improvement of hindlimb in rats was seen in all cell transplanted groups;NSCs group,OECs group and co-transplantation group demonstrated a significant increase than that of control group at 3 and 4 weeks post operation(P 0.05).Conclusions The engrafted NSCs,OECs and co-transplantation can improve partly the motor function recovery of hindlimb in rats at 3 to 4 weeks following cord transection;The OECs group and co-transplantation have better effects than that of NSCs group,but there is no obvious difference between the OECs group and co-transplantation group at observed time point in this investigation.
