Determination of the Content of Gentamycin Sulfate C Component in Medicinal Albumin Gel by HPLC
- VernacularTitle:HPLC法测定医用生物蛋白胶中硫酸庆大霉素C组分的含量
- Author:
Ping ZHANG
;
Yadong GUO
- Publication Type:Journal Article
- Keywords:
HPLC;
Medicinal albumin gel;
Gentamycin sulfate C component;
Determination of content
- From:
China Pharmacy
2007;0(34):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for the determination of gentamycin sulfate C component in medicinal albumin gel. METHODS: Samples was separated on Agilent ZORBAX Eclipse XDB-C8 at a column temperature of 26℃. The mobile phase was methanol-acetic acid-water (70∶5∶25, added with 0.02mol?L-1 sodium heptanesulfonate solution) for gradient elution at a flow rate of 1.0mL?min-1. The detection wavelength was set at 330nm. RESULTS: Baseline separation of gentamycin sulfate C component in medicinal albumin gel was achieved. The linear range of gentamycin sulfate C component was 0.26?g～6.50?g and its average recovery rate was 90.0%, RSD=0.43%, the lowest limit of detection was 0.368ng. CONCLUSION: The method was proved to be simple, accurate and reliable, and suitable for the content determination of gentamycin sulfate C component in related preparations.
