Identification of Scutellaria amoena CH Wright and Scutellaria baicalensis Georgi with HPLC Finger-prints
- VernacularTitle:HPLC指纹图谱鉴别滇黄芩与黄芩
- Author:
Chunmei XIONG
;
Ping ZHANG
;
Yadong GUO
- Publication Type:Journal Article
- Keywords:
Scutellaria amoena CH Wright;
Scutellaria baicalensis Georgi;
HPLC Fingerprints;
Identification
- From:
China Pharmacy
2007;0(33):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To identify different origin of Scutellaria amoena CH Wright and Scutellaria baicalensis Georgi indexed in China Pharmacopeia by HPLC fingerprints.METHODS:RP-HPLC method was adopted in which the samples were separated on Zorbax SB-C18(250mm?4.6mm,5?m)column at a column temperature of 30℃.The gradient elution was performed on the mobile phase which consisted of acetonitrile(contairing 1.0% methanoic acid)-water(containing 1.0% methanoic acid)at a flow rate of 1.0mL?min-1.The detection wavelength was set at 274nm.RESULTS:The fingerprints of Scutellaria amoena CH Wright that from different places were of great resemblance,so were the fingerprints between Scutellaria baicalensis Georgi that from different places,but the fingerprints between Scutellaria amoena CH Wright and Scutellaria baicalensis Georgi were of significant difference,with resemblance of only 0.859.CONCLUSION:The RP-HPLC fingerprint serves as basis for the further study of the quality criteria of Scutellaria amoena CH Wright and Scutellaria baicalensis Georgi.
