Determination of in Vitro Drug Release of Baicalin Liposomes by HPLC
- VernacularTitle:RP-HPLC法测定黄芩苷脂质体的体外释放度
- Author:
Shuo LIU
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Baicalin;
Liposomes;
In vitro release
- From:
China Pharmacy
2007;0(33):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for the determination of in vitro drug release of Baicalin liposomes.METHODS:The separation was performed on a C18 column(150mm?4.6mm,5?m)at 25℃;the mobile phase was composed of methanol-water-phosohoric acid(47∶53∶0.2)at a flow rate of 1.0mL?min-1.The ultraviolet wavelength was set at 280nm and the sample size was 10?L.RESULTS:The calibration curve showed good linearity in the range of 1.01～80.80?g?mL-1(r=0.999 8)and the average recovery rate was 100.05%(RSD=0.93%).CONCLUSION:The method is sensitive,simple and accurate,and applicable for the study of in vitro drug release of Baicalin liposomes.
