Quantitative Determination of Esculetine in Dinglian Tablets by RP-HPLC
- VernacularTitle:RP-HPLC法测定丁莲片中秦皮乙素的含量
- Author:
Fanghua ZHANG
;
Chao LI
;
Qixue TIAN
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Dinglian tablets;
Esculetine;
Content determination
- From:
China Pharmacy
2007;0(33):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a RP-HPLC method for the determination of Esculetine in Dinglian tablets.METHODS:The chromatographic column was Hypersil ODS C18(200mm?4.6mm,5?m)at a temperature of 25℃;the mobile phase was methyl-water-glacial acetic acid(40∶60∶0.4)at a flow velocity of 1.0mL?min-1,and the detection wavelength was set at 353nm.RESULTS:The linear concentration range of Esculetine was within 0.045～0.360?g(r=0.999 5).The average recovery rate was 97.72%(RSD=1.87%,n=5).CONCLUSION:This procedure is simple,accurate and reliable,and it can be used for the quality control of Dinglian tablets.
