Quantitative Determination of Geniposide in Zhibo Mixture by HPLC
- VernacularTitle:HPLC法测定栀柏合剂中栀子苷的含量
- Author:
Wenwu SUN
- Publication Type:Journal Article
- Keywords:
HPLC;
Zhibo mixture;
Geniposide;
Content determination
- From:
China Pharmacy
2007;0(33):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for the quantitative determination of Geniposide in Zhibo mixture.METHODS:The separation of sample was performed on Shim-pack VP-ODS C18 column(150mm?4.6mm,5?m)at a temperature of 25℃.The mobile phase consisted of acetonitrile-water(12∶88)at a flow rate of 1mL?min-1.The detection wavelength was set at 238nm.RESULTS:The linear range of Geniposide was 25～100mg?L-1(r=0.999 7).The average recovery was 99.89%(RSD=0.80%,n=9).CONCLUSION:The method was proved to be sensitive,specific,reproducible and suitable for quality control of Zhibo mixture.
