Effect of astragaloside(黄芪甲苷) on the expression of nuclear factor?? injured by hypoxia/reoxygenation in vascular endothelial cells
- VernacularTitle:黄芪甲苷对缺氧/复氧损伤血管内皮细胞核转录因子-?B表达的影响
- Author:
Fuguo YANG
;
Gexin LIU
;
Li WANG
- Publication Type:Journal Article
- Keywords:
astragaloside;
endothelial cells;
hypoxia/reoxygenation;
intercellular adhesion molecule-1;
nuclear factor??
- From:
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the intervention effect of astragaloside on the expression of nuclear factor??(NF-?B) injured by hypoxia/reoxygenation(H/R) in vascular endothelial cells.Methods: The human umbilical vein endothelial cells(hUVECs) were cultured and passaged.After the third generation,hUVECs were randomly divided into three groups: control group,H/R group and astagaloside group.The cells in the control group were cultured under normal conditions;the cells of H/R group were cultured under hypoxia,in a closed container with oxygen concentrations below 1% for 1 hour,and then under normal conditions for 1 hour in a CO2 foster box;the hUVECs in astragaloside group were pretreated by different dose of astragaloside(the final concentrations of 20,40,80 mg/L) at the time of pre-hypoxia,after 12 hours of the pretreatment,they suffered from H/R.The concentration of intercellular adhesion molecule-1(ICAM-1) and content of malondialdehyde(MDA) in supernatant were detected respectively by ABCenzyme linked immunosorbent assay(ELISA) and thio-barbituric acid method.The expression of NF-?? of the vascular endothelial cells was analyzed by immunohistochemistry.Results: Compared with the control group,the content of MDA and concentration of ICAM1 in the supernatant fluid were remarkably increased in the H/R group(MDA content(6.98?1.15) ?mol/L vs.(2.38?0.49) ?mol/L,ICAM-1 concentration(3 169.01?132.75) ng/L vs.(995.14?74.93) ng/L,both P
