Determination of Glycyrrhizic Acid in Jiaweihuoxiangzhengqi Pill by HPLC
- VernacularTitle:HPLC法测定加味藿香正气丸中甘草酸的含量
- Author:
Li LI
- Publication Type:Journal Article
- Keywords:
HPLC;
Jiaweihuoxiangzhengqi pill;
Glycyrrhizic acid;
Content determination
- From:
China Pharmacy
2007;0(33):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for the determination of Glycyrrhizic acid in Jiaweihuoxiangzhengqi pill.METHODS:The analysis was performed on a Varian C18(250mm?4.6mm,5?m)column at room temperature with a mobile phase consisted of acetonitrile-methanol-0.2mol?L-1 ammonium acetate-acetate(56∶9∶34∶1)at a flow rate of 0.9mL?min-1.The detection wavelength was set at 254nm.RESULTS:Good linear relationship was achieved when the detection concentration range of Glycyrrhizic acid was 10～50?g?mL-1(r=0.999 5).The average recovery was 99.06%(RSD=0.58%,n=9).CONCLUSION:The method was simple,rapid,sensitive and reproducible,which can be used for the quality control of Jiaweihuoxiangzhengqi pill.
