Determination of Concentration of Irbesartan in Human Plasma by HPLC with Fluorescence Detection
- VernacularTitle:HPLC-荧光检测法测定人血浆中厄贝沙坦的浓度
- Author:
Wangang WANG
;
Qiming LIU
- Publication Type:Journal Article
- Keywords:
HPLC with fluorescence detection;
Irbesartan;
Plasma concentration
- From:
China Pharmacy
2007;0(32):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine plasma concentration of irbesartan by HPLC with fluorescence detection.METHO-DS:Samples were separated on Diamonsil C18 with mobile phase consisted of 0.02mol?L-1 KH2PO4-acetonitrile(50∶50) at a flow rate of 1.0mL?min-1.The fluorescence detector was set at an excitation wavelength of 250nm and an emission waveleng-th of 375nm.The column temperature was 40℃.RESULTS:The linear range for irbesartan was from 1.0 to 1000ng?mL-1 (r=0.999 8).The mean relative recoveries of irbesartan at low,middle and high concentrations(2.0,50.0,and 500.00ng? mL-1) were 97.4%,106.1% and 101.6%,respectively.Both intra-day and inter-day RSD were below 10%.CONCLUSION:The method is simple,rapid,accurate and specific,and it can be used for the plasma concentration determination and pharmacokinetic study of irbesartan.
