Effects of Vasopressin on Genes Involve in Vesicle-mediated Transport in the Rat Inner Ear
- VernacularTitle:加压素对大鼠内耳囊泡介导转运有关基因的影响
- Author:
Fengming GU
;
Lianshan ZHANG
- Publication Type:Journal Article
- Keywords:
Vasopressin;
Inner ear;
Endolymphatic hydrops;
Vesicle-mediated transport;
cDNA microarray
- From:
Journal of Audiology and Speech Pathology
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of vasopressin on genes involve in vesicle-mediated transport in the rats' inner ear and to identify the possible mechanism of hydrolabyrinth induced by vasopressin.Methods 16 Wistar rats were divided into experimental group and controled group.Wistar rats of experimental group were intraperitoneally injected with 50 ?g/kg arginine vasopressin once a day for one week.Rats of controled group were injected with the same doses of physiologic saline.Then bullae both groups were immediately dissected out for total RNA extraction.cDNA were synthesized from total RNA of the inner ear by reverse transcription and labeled with Cy3 or Cy5.The BiostarR-40s cDNA microarray was hybridized with the above cDNA.The changes of mRNA expression intensity in the rats' inner ear induced by vasopressin injection were analyzed.Results Seven differentially expressed genes related to vesicle-mediated transport were screened out in the rat inner ear induced by vasopressin injection by use of cDNA microarray.Ap2b1,Arf1,Arf6,Dnch1,Mlc3 and Rab7 were increased by 2-fold or more,and Stx7 was decreased to 0.5-fold or less.Conclusion Vasopressin may disturb fluid homeostasis in the inner ear via vesicle-mediated transport resulting in hydrolabyrinth.
