Effect of CHOP/GADD153 on cardiomyocyte apoptosis induced by angiotensin Ⅱ in vitro
- VernacularTitle:CHOP/GADD153在血管紧张素Ⅱ诱导心肌细胞凋亡中的表达及作用
- Author:
Xiaojing ZOU
;
Le YANG
;
Shanglong YAO
- Publication Type:Journal Article
- Keywords:
Protein CHOP/GADD153;
Angiotensin Ⅱ;
Cardiomyocytes;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the relationship between alteration of CHOP/GADD153 protein expression and cardiomyocyte apoptosis induced by angiotensin Ⅱ (AngⅡ), and inhibitory effects of CHOP/GADD153 antisense oligodeoxynucleotide (anti-ODN) on apoptosis in vitro.METHODS: Cultured neonatal cardiomyocytes were exposed to AngⅡ with or without preincubation of CHOP/GADD153 anti-ODN. Variability of myocytes was measured by MTT assay, the lactate dehydrogenase (LDH) release was also detected, the percentage of annexin V positive myocytes was monitored by flow cytometry as apoptosis rate, CHOP/GADD153, Bcl-2 and Bax expressions were determined by Western blotting. RESULTS: Compared with control group, the expression of CHOP/GADD153 was obviously increased from (0.20?0.02 to 0.75?0.06) in AngⅡ group (P
