Determination of Vitamin D_2, Vitamin A and Vitamin E in Cernevit-12 for Injection by HPLC
- VernacularTitle:HPLC法测定注射用12种复合维生素中维生素D_2、维生素A与维生素E的含量
- Author:
Jinchun SONG
;
Junfen ZENG
- Publication Type:Journal Article
- Keywords:
HPLC;
Vitamin D2;
Vitamin A palmitate;
Vitamin E acetic ester;
Content determination
- From:
China Pharmacy
2007;0(31):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a HPLC method for the simultaneously determination of Vitamin D2, Vitamin A and Vitamin E in Cernevit-12 for injection. METHODS: An Inertsil(ODS-2) C18 column was selected as separation column at 30℃. The mobile phase consisted of acetonitrile-methanol-methylene chloride (80∶10∶10) at a flow rate of 1.0mL?min-1. The detection wavelength was set at 265nm and the injection volume was 10?L. RESULTS: The linear ranges of Vitamin D2, Vitamin E acetic ester and Vitamin A palmitate were 0.61～1.43?g?mL-1, 222.6～519.4?g?mL-1 and 1.21～2.82mg?mL-1 respectively. The average recoveries were 99.79%, 99.53% and 101.63%, with RSD of 1.66%, 0.184 6%, 0.113 4%. CONCLUSION: The method is simple, rapid and accurate, and suitable for the determination of Cernevit-12.
