Effects of steroid hormone on the expression of cyclic nucleotide-gated channels of olfactory receptor neurons
- VernacularTitle:地塞米松对嗅感觉神经元环核苷酸门控通道mRNA表达的影响
- Author:
Cong ZHANG
;
Yongxiang WEI
;
Xutao MIAO
;
Xiaochao LIU
;
Feihong XING
;
Hong ZHAO
;
Demin HAN
- Publication Type:Journal Article
- Keywords:
Glucocorticoids;
Olfactory Receptor Neurons;
Cyclic Nucleotide-Regulated Protein Kinases;
Ion Channels
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To observe the effects of Dexamethasone on the expression of cyclic nucleotide-gated channels (CNG channels) mRNA of olfactory receptor neurons (ORNs) by real-time quantitative reverse transcription-polymerase chain reaction(RT-PCR). METHODS Forty Wistar rats were randomly divided into four groups: 24-hours Dexamethasone treated group and its control group; 2-weeks Dexamethasone treated group and its control group. Dexamethasone was injected i.p. (1 mg/kg for 24-hours group, 0.2 mg/d for 2-weeks group). Control group rats were injected with the same volume of normal saline. Real-time quantitative RT-PCR was performed to evaluate mRNA production of CNGA2 subunits. RESULTS In Dexamethasone-injected rats, the up-regulation of CNGA2 mRNA was observed in 2-weeks group(P
