Arachidonic acid cytochrome P450 epoxygenases protect vessel endothelial cells against apoptosis induced by tumor necrosis factor-?
- VernacularTitle:细胞色素P450花生四烯酸表氧化酶抑制TNF-?诱导的内皮细胞凋亡
- Author:
Li LIN
;
Hong WANG
;
Yan WANG
;
Yan WANG
;
Zaiying LU
;
Daowen WANG
- Publication Type:Journal Article
- Keywords:
Endothelium-derived hyperpolarizing factor;
Endothelial cells;
Epoxyeicosatrienoic acids;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate whether endogenous endothelium-derived hyperpolarizing factors(EHDFs) produced by CYP epoxygenases BM3?F87V,2C11OR or CYP2J2 transfection was able to protect endothelial cells against apoptosis induced by tumor necrosis factor alpha.METHODS: Three or four passages of cultured bovine aortic endothelial cells(BAECs) were transfected with epoxygenases or the empty vector(pCB6).Cell viability was detected by MTT assay.Apoptosis of transfected endothelial cells was evaluated by DNA ladder assay,flow cytometry and morphological observations under fluorescence microscopy.RESULTS: Overexpression of CYP epoxygenases BM3?F87V,2C11OR,CYP2J2 increased cell viability respectively observed by MTT assay.The percentage of cells undergoing apoptosis was decreased in 2C11OR-,BM3F87V-or 2J2-transfected cells compared to the vector as evaluated by DNA fragment assay,flow cytometry analysis and morphological observations under fluorescence microscopy.CONCLUSION: Overexpression of CYP epoxygenases BM3?F87V,2C11OR or 2J2 increases cell viability and protects endothelial cells against TNF-?-induced apoptosis.These findings suggest new targets to investigate the endothelium-associated disorders and provide novel therapeutic strategies to treat them by modulating cytochrome P450 epoxygenases.
