Effect of VEGF on proliferation and differentiation of neural stem cells from the hippocampal gyrus of rat in vitro
- VernacularTitle:VEGF对大鼠海马回神经干细胞体外增殖和分化的影响
- Author:
Ying GUO
;
Dejin SHI
;
Chaofeng LIANG
;
Hui WANG
;
Wensheng LI
;
Zhuopeng YE
;
Liping HU
;
Yan LI
- Publication Type:Journal Article
- Keywords:
Neural stem cells;
Vascular endothelial growth factor;
Hippocampus
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effects of vascular endothelial growth factor(VEGF) on the proliferation and differentiation of neural stem cells(NSCs) of rats in vitro.METHODS: NSCs isolated from the hippocampal gyrus of SD rats were primary cultured and subcultured,and then divided into two groups:(1) the cells in VEGF group were treated with 150 ?g/L VEGF in the culture system,and VEGF was removed at the 7 th day;(2) control group(without VEGF treatment).The cellular morphology of two groups was observed by contrast phase microscope.Nestin and NF-200 expressing cells were detected via immunofluorescence method.The percentages of the immunostaining positive cells in each group at the 7 th day and at the 11 th day were determined.RESULTS: At the 7 th day,the percentage of nestin positive cells in VEGF group was 52.19%?7.95%,vs 29.26%?4.12% in control group(P