Effect of VCAM-1 modified human umbilical cord blood stromal cells on expansion of hematopoietic stem/progenitor cells
- VernacularTitle:VCAM-1修饰人脐血基质细胞对造血干/祖细胞扩增作用的实验研究
- Author:
Lei GAO
;
Xinghua CHEN
;
Xi ZHANG
;
Peiyan KONG
;
Xiangui PENG
;
Lin LIU
;
Hong LIU
;
Yao LIU
- Publication Type:Journal Article
- Keywords:
Vascular cell adhesion molecule-1;
Fetal blood;
Stromal cells;
Transfection;
Expanding
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of vascular cell adhesion molecule-1(VCAM-1) modified human umbilical cord blood stromal cells(hUCBSCs) on the expansion of hematopoietic stem cells/progenitor cells in vitro.METHODS: After the VCAM-1-pcDNA3.1(+) eukaryotic expression vector was constructed successfully,the recombinant vector was transfected into hUCBSCs via liposome mediation.The expression of the VCAM-1 was detected by semiquantitative RT-PCR and immunocytochemistry.The expansion abilities of hematopoietic cell in vitro under different conditions were compared,including cytokine stimulation,hUCBSCs feeder layer and VCAM-1 modified hUCBSCs feeder layer etc.The effect on hematopoietic reconstitution of different expanded hematopoietic cell groups was compared after CFU-GM,CFU-E and CFU-Mg semi-solid culture were proceed,respectively.RESULTS: The positive clone and NeoR gene detection proved that recombinant vector had been transfected into hUCBSCs.The results of semiquantitative RT-PCR and immunocytochemistry manifested that VCAM-1 was high expressed not only at transcriptional level in the stable-transfected hUCBSCs(P
