Effects of hydrogen peroxide on cyclooxygenase-2 expression in pulmonary arterial endothelial cells and the role of CaMKⅡ
- VernacularTitle:过氧化氢对肺动脉内皮细胞环氧合酶-2表达的影响及CaMKⅡ的作用
- Author:
Liping ZHU
;
Si JIN
;
Yuan SU
;
Dixun WANG
- Publication Type:Journal Article
- Keywords:
Ca2+-calmodulin dependent protein kinase;
Hydrogen peroxide;
Cyclooxygenase
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate if hydrogen peroxide may alter COX-2 gene expression in pulmonary artery endothelial cells(PAECs) and how CaMKⅡ functions in this process.METHODS: Cultured pulmonary arterial endothelial cells were treated with different concentrations of hydrogen peroxide for different durations.The cells survival rates were measured by CCK-8 after the cells were treated by hydrogen peroxide.The level of COX-2 mRNA and protein were measured by RT-PCR and Western blotting,respectively.RESULTS: The results showed that hydrogen peroxide up-regulated COX-2 mRNA and protein levels in a concentration-and time-dependent manners.Incubation with 100 ?mol/L H2O2 for 4 h increased COX-2 mRNA and protein level to 256.01%?22.36%(P
