Effects of the calpain system expression on atrial structural remodeling in canine with atrial fibrillation
- VernacularTitle:钙激活蛋白酶系统与慢性房颤犬心房重构关系的研究
- Author:
Xiao MA
;
Wei ZHANG
;
Ming ZHONG
;
Li LI
;
Ya MIAO
;
Hui SUN
;
Yun ZHANG
- Publication Type:Journal Article
- Keywords:
Atrial fibrillation;
Calpain;
Canine
- From:Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To evaluate the influence of the calpain system mRNA and protein expression on the progress of atrial structural remodeling in fibrillating canine.METHODS: 17 dogs were randomly divided into 2 groups: normal control group(SR,n=6) and atrial fibrillation(AF,n=11) group.AF was induced by rapid pacing for 8 weeks and all dogs underwent transthoratic echocardiography before and after rapid pacing.The mRNA and protein expression of calpainⅠ,calpainⅡand calpastatin were assessed by real-time quantitative PCR and Western blotting,respectively.RESULTS: Compared with SR group,the left atrial diameters and the content of calcium in atrial myocardium increased significantly in AF group(P0.05) between two groups.The expression of calpastatin mRNA was upregulated significantly in AF group(P
