Protective effect of wortmannin against pancreas and liver injuries induced by severe acute pancreatitis in rats
- VernacularTitle:wortmannin对重症急性胰腺炎大鼠胰肝损伤的保护作用
- Author:
Bingsuo LI
;
Wenke CAI
;
Yanshun REN
;
Junqiang DANG
;
Hongbing ZHOU
;
Kefeng DOU
;
Qingchuan ZHAO
- Publication Type:Journal Article
- Keywords:
Pancreatitis, Acute Necrotizing;
wortmannin;
NF-?B;
liver injuries
- From:
Chinese Journal of General Surgery
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effect of pretreatment with wortmannin against pancreas and liver injuries indued by severe acute pancreatitis(SAP) in rats and investigate its mechanism.Methods Fifty-four SD rats were randomly divided into 3 groups: control group(C group),SAP group(P group) and SAP+wortmannin group(PW group)(n=18 per group). SAP model was induced by retrograde infusion of 50g/L sodium taurocholate into the biliopancreatic duct of rats,except C group in which sodium taurocholate was replaced by normal saline. Serum level of tumor necrosis factor-alpha(TNF-?)、 alanine aminotransferase(ALT)、aspartate aminotransferase(AST)、and nuclear factor-kappa B(NF-?B)in liver were detected. Histopathology of liver and pancreas was studied.Results In P group, serum levels of TNF-?、ALT、AST and NF-?B in liver were significantly elevated(P
