Determination of Yohimbine in Corynanthe Yohimbe K. Schum. by HPLC
- VernacularTitle:HPLC法测定育亨宾树皮中育亨宾的含量
- Author:
Qinhua CHEN
;
Jun ZHU
;
Kaijun LI
- Publication Type:Journal Article
- Keywords:
HPLC;
Corynanthe yohimbe K.schum.;
Yohimbine;
Content determination
- From:
China Pharmacy
2007;0(28):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for the determination of yohimbine in corynanthe yohimbe K.schum.METHODS:The analysis was performed on C18 column with column under room temperature.The mobile phase consisted of methanol-phosphoric acid buffer-triethylamine(30∶ 70∶ 0.5,pH5.0)with a flow rate of 1.0mL? min-1.The wavelength was set at 270nm.RESULTS:There was a good linear relationship when the sample size of yohimbine was over a range of 10～ 1 000? g? mL-1(r=0.999 8),with recovery at 96.5% and precision at 1.23%.CONCLUSION:This method was simple and reliable,and suitable for the determination of content of yohimbine.
