Induction of Apoptosis by Tanshinone Ⅱ_A in HeLa Cells
- VernacularTitle:丹参酮Ⅱ_A对HeLa宫颈癌细胞凋亡的影响
- Author:
Jun SHEN
;
Lili DU
;
Aimin ZHAI
- Publication Type:Journal Article
- Keywords:
Tanshinone Ⅱ_A;
HeLa cells;
Apoptosis
- From:
China Pharmacy
2007;0(27):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To discuss the effect of tanshinone Ⅱ A on the apoptosis of HeLa cell line and its mechanism.METHODS:MTT was used to measure the levels of the proliferation of HeLa cells cultured with tanshinone Ⅱ A in different concentrations.The apoptosis was measured by Hoechst33258 staining,and the level of mRNA of Bcl-2 and bax in HeLa cells was tested by semi-fix quantify RT-PCR method.RESULTS:Tanshinone Ⅱ A could inhibit the proliferation of HeLa cells in a dose dependent manner,induce cell apoptosis,and markedly decrease the expression of Bcl-2 on mRNA level after treatment for 48h,while it had no obvious change in bax mRNA level.CONCLUSION:Tanshinone Ⅱ A can inhibit the proliferation of HeLa cells and induce the apoptosis of the cells.
