Effects of AP-1 element on endothelin-1 gene transcription in homocysteine-induced HUVECs
- VernacularTitle:EDN1基因5′上游AP-1顺式调控元件在同型半胱氨酸诱导HUVECs EDN1基因转录中的作用
- Author:
Jinhong DUAN
;
Haishan XU
;
Shunling DAI
;
Xiaoming WANG
;
Yunqing WU
;
Yandong ZHANG
;
Jinxuan CHENG
;
Renyu SUN
;
Liang LI
;
Huaiqing CHEN
- Publication Type:Journal Article
- Keywords:
Transcription factor AP-1;
Homocysteine;
Genes,EDN1
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:The objective of this study was to observe the effects of AP-1 element on endothelin-1(EDN1)gene transcription in homocysteine(Hcy)-induced human umbilical vein endothelial cells(HUVECs).METHODS:The redox level in Hcy-induced HUVECs was determined by using the fluorescent product DCF.The influences of Hcy on expressions of EDN1 mRNA and protein of c-jun/AP-1 in HUVECs were measured by the methods of RT-PCR and Western blotting,respectively.The EDN1 secretion level of HUVECs induced by Hcy was determined by enzymatic immunoassay.The transient transfection assay was performed and luciferase activity of transcriptional factor AP-1 reporter gene induced by Hcy was detected.RESULTS:The Hcy significantly increased EDN1 secretion,EDN1 mRNA expression and oxidative stress in HUVECs.Hcy remarkably induced the expression of pGL3-EDN1-AP-1(115/+135)luciferase reporter gene plasmid.However,basic expression of mutation of pGL3-EDN1-AP-1(-115/+135)luciferase reporter gene plasmid was downregulated markedly in HUVECs induced by Hcy.CONCLUSION:Redox-active and release of ROS are changed by Hcy.Activated AP-1 element plays an important role in EDN1gene transcription in HUVECs induced by Hcy.
