Effect of nuclear factor kappa B on TNF-? induced proliferation,apoptosis and the expression of TGF-?_1 in airway smooth muscle cells
- VernacularTitle:核因子-?B对TNF-?诱导的大鼠气道平滑肌细胞增殖、凋亡及TGF-?_1表达的影响
- Author:
Biwen MO
;
Zhenxiang ZHANG
;
Yongjian XU
;
Changming WANG
;
Weining XIONG
;
Xiansheng LIU
;
Chunsheng FANG
- Publication Type:Journal Article
- Keywords:
Airway smooth muscle cells;
NF-kappa B;
Cell proliferation;
Apoptosis;
Transforming growth factor beta
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of nuclear factor kappa B(NF-?B)on proliferation,apoptosis and the expression of TGF-?1 in airway smooth muscle cells(ASMCs).METHODS:Cultured ASMCs were divided into three groups and stimulated with or without TNF-? and pyrrolidine dithiocarbamate(PDTC)in vitro.Reverse transcriptase-polymerase chain reaction(RT-PCR)was used to detect the expression of TGF-?1 mRNA.The location and protein expression of PCNA and Bcl-2 were observed by immunocytochemical staining.The protein expression of TGF-?1 and NF-?B was detected by Western blotting.The proliferation and apoptosis of ASMCs were also observed.RESULTS:The activity of NF-?B in TNF-? group was significantly higher than that in control group and TNF-? plus PDTC group,respectively(P
