Expression and significance of thrombospondin-1 in myocardium of type 2 diabetic cardiomyopathy rats
- VernacularTitle:凝血酶敏感蛋白-1在2型糖尿病心肌病大鼠心肌中的表达及意义
- Author:
Ming ZHONG
;
Wei ZHANG
;
Ya MIAO
;
Peili BU
;
Hui SUN
;
Xiao MA
;
Huiping GONG
;
Mengxiong TANG
;
Yun ZHANG
- Publication Type:Journal Article
- Keywords:
Diabetic cardiomyopathy;
Thrombospondin 1
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the expression and significance of thrombospondin-1(TSP-1)in left ventricular myocardium of type 2 diabetic cardiomyopathy(DCM).METHODS:The rat model of DCM was established by eating a high-fat diet together with injection of low dose streptozocin(30 mg/kg)intrapertoneally.After 12 weeks,the content of collagen was quantified by Masson staining.The mRNA level of TSP-1 was determined by quantification real-time RT-PCR,while the protein level of TSP-1 was analyzed by Western blotting and immunohistochemistry.RESULTS:Compared with the control group,the content of collagen in the DCM group was increased greatly(11.01?3.05 vs 16.92?3.18,P
