Effect of recombinant adenovirus vector of decorin on the expression of extracellular matrix molecules in rat mesangial cells
- VernacularTitle:Decorin基因转染抑制高糖培养的大鼠肾系膜细胞细胞外基质mRNA表达
- Author:
Feng ZHU
;
Hong LI
;
Fang WU
;
Zhenggang YANG
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathies;
Decorin;
Transforming growth factor beta;
Extracellular matrix;
Gene transfection
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of adenovirus-medicated decorin gene transfer into rat mesangial cells(RMCs)on the expression of decorin,TGF-?1 and the extracellular matrix(ECM)components.METHODS:Rat mesangial cells were transfected with recombinant adenovirus vector of decorin,the levels of mRNA for decorin,TGF-?1,collagen type IV and fibronectin were estimated by semi-quantitative RT-PCR.RESULTS:The transfection of AD-DCN significantly increased decorin mRNA expression compared with negative control(P
