Expression of connective tissue growth factor in renal interstitial fibrosis after unilateral ureteral obstruction in rats
- VernacularTitle:结缔组织生长因子在单侧输尿管梗阻大鼠肾脏中的表达及其意义
- Author:
Chun ZHANG
;
Zhonghua ZHU
;
Jianshe LIU
;
Xiao YANG
;
Anguo DENG
- Publication Type:Journal Article
- Keywords:
Connective tissue growth factor;
Transforming growth factor beta;
Collagen type Ⅰ;
Plasminogen activator inhibitor 1;
Renal fibrosis
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the expression of connective tissue growth factor(CTGF)in unilateral ureteral obstruction(UUO)rats,and to explore its pathogenic role in renal tubulointerstitial fibrosis.METHODS:48 Wistar rats were randomly divided into sham-operated and UUO group.The rats were sacrificed at day 1,3,7,and 14.The degree of tubulointerstitial damage was scored according to the Masson staining.The mRNA and protein levels of CTGF,transforming growth factor-?1(TGF-?1),collagen Ⅰ(Col Ⅰ),and plasminogen activator inhibitor-1(PAI-1)were detected by reverse transcriptional-polymerase chain reaction(RT-PCR)and immunohistochemistry,respectively.Expression of CTGF protein in the kidney was also assessed using Western blotting.RESULTS:TGF-?1 mRNA level began to increase as early as 1 day after UUO.This increase was followed by the elevation of CTGF mRNA level,which began to increase at third days after UUO(P
