Gene expression of spleen lymphocytes induced by gp96-peptide complexes and its anti-tumor effect in vitro
- VernacularTitle:gp96-肽复合物体外对脾淋巴细胞基因表达及其抗肿瘤效应研究
- Author:
Changlai ZHU
;
Lin LIN
;
Tianyi ZHANG
- Publication Type:Journal Article
- Keywords:
gp96;
Heat-shock proteins;
Spleen lymphocytes;
Apoptosis;
Gene expression
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the expressing variation of TNF-? and IFN-? mRNA in mouse splenocytes induced by H22 tumor cells derived heat shock protein gp96-peptide complexes in vitro,and to observe the morphologic change of H22 tumor cells which treated with the culture supernatant.METHODS:H22 tumor cells derived HSP gp96 was obtained by the techniques for protein extraction and purification and was identified by Western blotting method.The expression values of TNF-? and IFN-? mRNA in spleen lymphocytes were detected by semi-quantitative RT-PCR.The morphologic changes of H22 tumor cells induced by the culture supernatant were observed by laser scanning confocal microscopy(LSCM)and transmission electron microscope(TEM).RESULTS:Purified heat shock protein gp96 was identified by Western blotting.The expression value of TNF-? and IFN-? mRNA in activated spleen lymphocytes induced by gp96-peptide complexes was higher than that in control groups(P
