Expressions of methylthioadenosine phosphorylase gene and ornithine decarboxylase in ovarian cancer
- VernacularTitle:甲硫腺苷磷酸化酶基因和鸟氨酸脱羧酶在卵巢癌中的表达及意义
- Author:
Xiaoyu PAN
;
Bo WANG
;
Zhanping WENG
;
Hongying DAI
;
Lin XU
- Publication Type:Journal Article
- Keywords:
Ovarian neoplasms;
Methylthioadenosine phosphorylase;
Gene expression;
Ornithine decarboxylase
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the expressions of methylthioadenosine phosphorylase(MTAP)and ornithine decarboxylase(ODC)in human ovarian cancer.METHODS:60 fresh samples of ovarian cancer were collected.The expressions of MTAP mRNA and protein were analyzed by using RT-PCR and Western blotting,respectively.ODC activity was measured by high performance liquid chromatography.RESULTS:The expression levels of MTAP mRNA and protein in ovarian cancer were lower than those of control.In 9 of the 60 samples(15%)there were absence of detectable MTAP mRNA and protein.No significant relevance was found between the expression of MTAP and clinical pathologic features.ODC activity in ovarian cancer was(3.82?1.03)U,which was higher than that of normal ovarian tissues(1.38?0.59)U.ODC activity was related with tumor grade.In MTAP-deficiency ovarian cancer tissues ODC activity was significantly increased when compared with that of MTAP-expressing ovarian cancer samples.CONCLUSION:Down-regulated MTAP expression and up-regulated ODC activity really exist in ovarian cancer.Activation of ODC resulting from MTAP deletion may be one of the pathogenetic factors of ovarian cancer.
