Relationship between genetic instability of nm23H1 gene and clinical pathological characteristic in hepatocellular carcinoma
- VernacularTitle:肝细胞癌nm23H1基因遗传不稳定性与临床病理特征的相关性研究
- Author:
Haiying LU
;
Jicheng LI
;
Xiaoyue LIANG
;
Ling ZHANG
- Publication Type:Journal Article
- Keywords:
Liver neoplasms;
Genes,nm23H1;
Microstatellite instability;
Loss of heterozygosity
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:The aim of this study was to examine the microstatellite instability(MSI)and loss of heterozygosity(LOH)of locus D17S396 on chromosome 17 and their influence on the expression of nm23H1 in hepatocellular carcinoma(HCC),which may provide experimental evidence for the mechanism of nm23H1 gene and tumor metastasis.METHODS:Techniques such as DNA extraction from formalin-fixed paraffin-embedded tissues,PCR-SSCP,ordinary silver stain were used to study MSI and LOH of locus D17S396.Envision immunohistochemistry and Leica-Qwin computer imaging techniques were used to assess the expression of nm23H1.RESULTS:① The frequency of heredity instability of HCCs was 35.42%.The frequency of LOH in the cases with lymph node or distant organs metastasis or not and with intrahepatic metastasis or embolus of portal vein or not was significantly different(P
