Detection of peritoneal micrometastases of gastric cancer and its clinical significance
- VernacularTitle:胃癌腹腔微转移检测及其临床意义
- Author:
Wencai QIU
;
Weigang WANG
;
Zhigang WANG
;
Gang CHEN
;
Shifu XI
;
Qi ZHENG
- Publication Type:Journal Article
- Keywords:
Stomach Neoplasms/pathol;
Cytokeratin 20(CK-20);
Peritoneal Micrometastasis
- From:
Chinese Journal of General Surgery
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the clinical significance of the intraoperative detection of peritoneal micrometastases of gastric cancer.Methods In selected 50 cases of gastric cancer in which no obvious peritoneal metastasis was found preoperatively or during laparotomy,Douglas′s pouch peritoneal biopsy was undertaken intraoperatively,then HE and CK-20 immunohistochemistry staining of the specimens was performed.The expression of CK-20 mRNA in peritoneal irrigation fluid was also determined by RT-PCR.Results HE staining of all cases was negative.The positive rate of CK-20 immunohistochemistry staining was 24.0 %(12/50),and 36.0 %(18/50) with RT-PCR method.The positive rate of CK-20 mRNA was significantly related with the histological type,the depth of invasion and the number of lymph node metastasis(P
