siRNAs interference exogenous GFP gene expression in neuro-2a cells
- VernacularTitle:siRNA抑制外源绿色荧光蛋白在neuro-2a细胞株中的表达
- Author:
Xiaoqin ZHANG
;
Feng LI
;
Yan ZHAO
;
Yingji PENG
;
Yuchun PAN
;
He MENG
;
Fangyan CUI
- Publication Type:Journal Article
- Keywords:
RNA interference;
Green flulorescent protein;
Neurons
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To assess the effect of RNAi on suppressing the exogenous reporter gene expression in mammalian neurons,and explore the effect of siRNA quantitation on interference efficiency.METHODS: Exogenous green fluorescent protein(GFP) expression vector was transferred into neuro-2a cells,and then the small interference RNA targeting GFP mRNA(siGFP) synthesized by transcription in vitro at three different concentration was used in this experiment.RESULTS: The results showed that the neuro-2a cells can be transfected efficiently and siGFP can inhibit GFP expression greatly.CONCLUSION: RNAi can be applied into mammalian neurons successfully.The research on siRNA quantitation will provide technique support for studying the gene function of neurons in the future.
