Effects of different therapeutic methods and typical recipes on activation of ERK1/2 in Kupffer cells of rats with fatty liver
- VernacularTitle:不同治法方药对大鼠脂肪肝Kupffer细胞ERK1/2蛋白活性的影响
- Author:
Minjie MENG
;
Qinhe YANG
;
Qiang WANG
;
Xuemei CHEN
;
Fengzhen WANG
;
Yanping WANG
;
Hailan TANG
;
Shaobing CHENG
;
Jiasheng LING
;
Chengyuan WEN
;
Fang XIE
- Publication Type:Journal Article
- Keywords:
Fatty liver;
Rats;
Mitogen-activated protein kinases;
Drugs,Chinese herbal
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate effects of different therapeutic methods and typical recipes on activation of ERK1/2 in Kupffer cells of rats with fatty liver.METHODS: The rat model of fatty liver was established by feeding high fat diet combinated with distillate spirit.Meanwhile Chinese medicines Shugan fang,Jianpi fang,Huoxue fang,Qushi fang,and Zonghe fang were given to treat different groups respectively.12 weeks later,the Kupffer cells were isolated from livers of control group,model group and different treatment groups by sequential in situ perfusion with collagenaseⅣ and pronase E,density gradient centrifugation,selective adherence.The expression of total ERK1/2 and phospho-ERK1/2 in Kupffer cells of control group,model group and different treatment groups were detected by Western blotting.RESULTS: The expressions of total ERK1/2 and phospho-ERK1/2 were higher in Kupffer cells from model group than those in control group(P
