Experimental study on how brain-dead state affects the heart structure and function of Ba-Ma mini pigs and the mechanism
- VernacularTitle:脑死亡对巴马小型猪心脏结构与功能的影响及其机制的实验研究
- Author:
Shuijun ZHANG
;
Shengxing ZHU
;
Jie LI
;
Xiuxian MA
;
Liushun FENG
;
Zhengjun FAN
- Publication Type:Journal Article
- Keywords:
Ba-Ma mini pigs;
Brain death;
Protein kinase C;
Heart injuries
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate how brain-dead state affects the heart structure and function and the effect of PKC-? in BA-Ma mini pigs.METHODS: Ten Ba-Ma mini pigs were randomized into 2 groups: brain-dead group(n=5),and control group(n=5).The brain-dead model was made by increasing intracranial pressure,while the control group was maintained anesthesia for 24 h.The concentrations of cTnT,TNF-?,IL-1? and IL-6 in serum were determined at 6,12 and 24 h after brain death.At 24 h,heart tissues were observed by HE staining and electron microscope.The expression of PKC-? was detected by immunohistochemistry and RT-PCR.RESULTS:(1) Histological changes of myocardium: flaky bleeding under endocardium and dissolution of myocardium were found in optical microscope.In electron microscope dropsical mitochondria and confluent muscle fiber were found.(2) Changes of serum cTnT: serum cTnT for brain-dead group began to increase gradually since 6 h,and were significantly higher at each time point than those in control group(P
