Determination of Cefoperazone Sodium in Cefoperazone Sodium/Sulbactam Sodium in Bile by HPLC
- VernacularTitle:HPLC法测定头孢哌酮钠/舒巴坦钠中头孢哌酮钠的胆药浓度
- Author:
Genying XIE
;
Chenghong JI
;
Jiubing GUO
;
Wenjun XU
;
Huiyi XU
- Publication Type:Journal Article
- Keywords:
HPLC;
Cefoperazone sodium;
Concentration of medicine in bile
- From:
China Pharmacy
2005;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the determination of the concentration of cefoperazone sodium in cefoperazone sodium / sulbactam sodium in bile by HPLC.METHODS:The bile specimen was collected from the gallbladder of patients during laparoscopic cholecystectomy and the concentration of cefoperazone sodium was determined by HPLC.The separation of cefoperazone sodium was performed on YWG C18 with column temperature at 35℃.The mobile phase consisted of methanol-water-glacial acetic acid(25.5∶74.5∶0.45) with flow rate at 1.0mL?min-1 and UV detection wavelength at 254nm.RESULTS:The linear range of ceforazone sodium in human bile was 1.25～62.5?g?mL-1(r=0.999 1),and its average recovery was 95.14%(RSD=3.42%).CONCLUSION:The above method is simple and reliable,and it can provide bases for clinical rational use of drugs.
