Study on induction of apoptosis of biliary duct cancer cells by ?-ray of the ~(103)Pd biliary stent
- VernacularTitle:~(103)钯胆管放射性支架释放的?射线诱导胆管癌细胞凋亡的研究
- Author:
Guijin HE
;
Dongming SUI
;
Faqiang YU
;
Dawei JI
;
Qinyi GAO
;
Xianwei DAI
;
Hong GAO
;
Tao JIANG
;
Chaoliu DAI
- Publication Type:Journal Article
- Keywords:
Bile Duct Neoplasms/rad eff;
?-ray radiation;
Apoptosis
- From:
Chinese Journal of General Surgery
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism and role of ?-ray of 103Pd in the treatment of biliary duct cancer.Methods A series of biliary duct cancer cells were treated with different ?-ray dose,and MTT [3-(4,5-dimethy thiazol-2-yl)-2,5-diphenyl terazolium-bromide] technique was used to determine the inhibition rate of ?-ray of 103Pd on the biliary duct cancer cells;and electron micro-technique,DNA agarose gel electrophoresis and flow cytometry to evaluate the morphological characteristics and apoptosis rate of the biliary duct cancer cells were also used.Results The ?-ray radiation of 103Pd resulted in significant inhibition of the biliary duct cancer cells.The features of biliary duct cancer cells apoptosis(e,g:apoptic bodies,DNA ladders band hypodiploid DNA peak) could be seen in the group with lower dosage(5.333mci),and cell necrosis was seen in higher dosage(more than 6.645 mci).Conclusions The ?-ray radiation could induce apoptosis of the biliary duct cancer cells,but with dose dependence,and apoptosis can be an important mechanism for radiation treatment of biliary duct cancer.
