Effect of Ginkgo biloba extract on cell proliferation and expression of TGF-1 and CTGF in rat hepatic stellate cells
- VernacularTitle:银杏叶提取物对肝星状细胞增殖及转化生长因子和结缔组织生长因子基因表达的影响
- Author:
Yuhua ZHU
;
Chunqing ZHANG
;
Hongwei XU
;
Wanhua REN
;
Xiaoxia L
- Publication Type:Journal Article
- Keywords:
Ginkgo biloba;
Hepatic stellate cells;
Transforming growth factor beta;
Connective tissue growth factor;
Gene exprerssion
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of Ginkgo biloba extract(EGb761) on cell proliferation and expression of transforming growth factor ?1(TGF-?1) and connective tissue growth factor(CTGF) in rat hepatic stellate cells(HSC-T6).METHODS:HSC-T6 was cultured with EGb761 at concentration of 1,10,100 and 500 mg/L,and without EGb761 as blank control.MTT colorimetric assay was used for detecting the proliferation of HSCs and flow cytometry was used for observing the cell cycles of HSCs under different concentrations of EGb761.The expression of TGF-?1/CTGF mRNA and protein levels were determined by reverse transcription-polymerase chain reaction(RT-PCR) and Western blotting after 24 hours and 48 hours.RESULTS:EGb761 inhibited the proliferation of HSCs at the concentration of 10 mg/L,100 mg/L and 500 mg/L.G0/G1 stage was increased(P
