Neuropeptide Y induced redistribution of intracellular free calcium in rat cardiomyocytes
- VernacularTitle:神经肽Y诱导大鼠心肌细胞内游离钙重分布研究
- Author:
Longgen XIONG
;
Yi ZHANG
;
Tao SU
;
Shiming LIU
;
Qi DONG
;
Minsheng CHEN
- Publication Type:Journal Article
- Keywords:
Neuropeptide Y;
Cardiomyocytes;
Sarcoplasmic reticulum;
Calcium
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of neuropeptide Y(NPY) on intracellular free calcium([Ca2+]i) and Ca2+ sarcoplasmic reticulum of cardiomyocytes in rats.METHODS:Cardiomyocytes of neonatal Sprague-Dawley rats were incubated with NPY at concentration of 100 nmol/L for 24 h.Fluorescent indicator Fluo-4 AM was used to detect [Ca2+]i and Fluo-5N AM was used to detect Ca2+ in sarcoplasmic reticulum(SR).Calcium image was recorded by laser scanning confocal microscope.The SR Ca2+ load was estimated by caffeine-induced Ca2+ transient(CCT).RESULTS:24 h after incubation with NPY,compared with control group,the concentration of [Ca2+]i was significantly elevated(P
